Ethnopharmacology of Indigofera tinctoria (Linn.): Bridging Traditional Knowledge and Evidence-Based Medicine
DOI:
https://doi.org/10.51574/hayyan.v3i1.5057Keywords:
Indigofera tinctoria, ethnopharmacology, phytochemistry, traditional medicine, pharmacological activityAbstract
Indigofera tinctoria (Linn.), commonly known as true indigo, is a medicinal plant traditionally used in various ethnomedical systems, including Ayurveda, Traditional Chinese Medicine (TCM), and African traditional medicine. Beyond its historical role as a natural dye source, the plant has attracted increasing scientific interest due to its diverse pharmacological properties and bioactive constituents. This review aimed to systematically evaluate the ethnopharmacological uses, phytochemical composition, and pharmacological activities of I. tinctoria, while highlighting its potential for evidence-based therapeutic applications. A narrative review with a systematic literature search approach was conducted using electronic databases, including Scopus, PubMed, Web of Science, Google Scholar, and ScienceDirect. Relevant studies published between 2020 and 2026 were identified using keywords related to Indigofera tinctoria, ethnopharmacology, phytochemistry, and pharmacological activity. Study selection followed PRISMA guidelines, and a total of 45 eligible studies were included in the qualitative synthesis. The findings demonstrated that I. tinctoria has been traditionally utilized for the treatment of inflammatory disorders, skin diseases, infections, liver dysfunction, fever, and metabolic abnormalities. Phytochemical analyses revealed the presence of flavonoids, alkaloids, tannins, saponins, terpenoids, phenolic compounds, indigo, and indirubin, which contribute to its biological activities. Experimental studies reported significant anti-inflammatory, antioxidant, antimicrobial, hepatoprotective, antidiabetic, and immunomodulatory effects. Among the identified compounds, indirubin showed notable pharmacological potential due to its anti-inflammatory and antiproliferative properties. However, the current evidence remains predominantly based on in vitro and animal studies, while clinical validation in humans is still limited. In conclusion, Indigofera tinctoria possesses promising ethnopharmacological and therapeutic potential supported by growing scientific evidence. Nevertheless, further studies focusing on standardized phytochemical characterization, mechanistic investigations, toxicological assessments, and randomized clinical trials are necessary to facilitate its translation into evidence-based phytotherapeutic applications.
References
Aierken, K., Li, J., & Waheed, A. (2025). Fritillaria pallidiflora Schrenk ex Fisch. & C.A.Mey. (Yi Beimu): Ethnopharmacology, Phytochemistry, Pharmacological Insights, and Future Prospects. Plants, 14(24), 1–25. https://doi.org/10.3390/plants14243771
Asaduzzaman, M., Nahar, L., Biswas, M. S., & Rahman, M. M. (2026). Molecular Mechanisms of Anti-Inflammatory and Immunomodulatory Activities of Key Phytochemicals: A Comprehensive Review. Journal of Tropical Medicine, 2026(1), 1–30. https://doi.org/10.1155/jotm/9906781
Babalola, O. O., Bridget, K., Oyubu, G., & Waheed, S. A. (2025). Integrating phytochemicals and in silico methods for modern drug discovery : a comprehensive review. Discover Chemistry, 2(297), 1–44. https://doi.org/https://doi.org/10.1007/s44371-025-00373-y
Bhosale, R., Shinde, S., & Chougule, N. (2024). Herbal Drug Formulation: Quality Aspects and Standards. Int. J. of Pharm. Sci, 2(12), 265–275. https://doi.org/10.5281/zenodo.14265998
Calderon Martinez, E., Ghattas Hasbun, P. E., Salolin Vargas, V. P., García-González, O. Y., Fermin Madera, M. D., Rueda Capistrán, D. E., Campos Carmona, T., Sanchez Cruz, C., & Teran Hooper, C. (2025). A comprehensive guide to conduct a systematic review and meta-analysis in medical research. Medicine (United States), 104(33), e41868. https://doi.org/10.1097/MD.0000000000041868
Chodok, P., Suweeranon, J., Khumkhom, S., Baipong, S., & Chaikham, P. (2025). Extraction of antioxidant compounds from Aeginetia indica flowers. Food Chemistry Advances, 9(July), 101117. https://doi.org/10.1016/j.focha.2025.101117
De Rossi, L., Rocchetti, G., Lucini, L., & Rebecchi, A. (2025). Antimicrobial Potential of Polyphenols: Mechanisms of Action and Microbial Responses—A Narrative Review. Antioxidants, 14(2). https://doi.org/10.3390/antiox14020200
Dubale, S., Usure, R. E., Mekasha, Y. T., Hasen, G., Hafiz, F., Kebebe, D., & Suleman, S. (2025). Traditional herbal medicine legislative and regulatory framework: a cross-sectional quantitative study and archival review perspectives. Frontiers in Pharmacology, 16(January), 1–22. https://doi.org/10.3389/fphar.2025.1475297
Duraisamy, P., Ravi, S., Krishnan, M., Martin, L. C., Manikandan, B., Raman, T., Munusamy, A., & Ramar, M. (2023). Scoparia dulcis and Indigofera tinctoria as potential herbal remedies against 7-ketocholesterol-induced pro-inflammatory mediators of macrophage polarization. Journal of Herbal Medicine, 39(March), 100652. https://doi.org/10.1016/j.hermed.2023.100652
Elmahaishi, L. M. S., Fisher, F., Hussein, A., & Africa, C. W. J. (2025). The Role of African Medicinal Plants in Dermatological Treatments: A Systematic Review of Antimicrobial, Wound-Healing and Melanogenesis Inhibition. Cosmetics, 12(4), 1–21. https://doi.org/10.3390/cosmetics12040132
Febriyanti, R. M., Ramadhania, Z. M., Tjitraresmi, A., Indradi, R. B., Maisyarah, I. T., Maundu, P. M., Muhaimin, M., & Sujarwo, W. (2026). A systematic review of ethnobotanical study in Indonesia: diversity and cultural patterns of medicinal plant use. Journal of Ethnobiology and Ethnomedicine, 22(1). https://doi.org/10.1186/s13002-026-00879-4
Gusenbauer, M., & Gauster, S. P. (2025). How to search for literature in systematic reviews and meta-analyses: A comprehensive step-by-step guide. Technological Forecasting and Social Change, 212(December 2024), 123833. https://doi.org/10.1016/j.techfore.2024.123833
Hassanpour, S. H., & Doroudi, A. (2023). Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna Journal of Phytomedicine, 13(4), 354–376. https://doi.org/10.22038/AJP.2023.21774
Hosain, Z., Palash, M. F. H., Kabiraj, A., Yasmin, S., Das, S., & Akter, S. (2024). Assessing Medicinal properties of Indigofera tinctoria extracts, post-fermented dye, and dyed fabric. Authorea, 1–13. https://www.authorea.com/doi/pdf/10.22541/au.171739784.47366700/v1
Kimta, N., Puri, S., Kumari, A., Choi, B. H., Nepovimova, E., Alomar, S., & Kuca, K. (2024). Impact of altitudinal variation on secondary metabolites, antioxidant, anti-inflammatory and anticancer potential of Adiantum venustum D. Don. Journal of Agriculture and Food Research, 18(June), 101297. https://doi.org/10.1016/j.jafr.2024.101297
L, V. S., Bhairavi, A., & Sathishkumar, R. (2025). Discovery of Bioactive Compounds From Medicinal Plants: Insights Into Wrightia Tinctoria as a Potential Antistaphylococcal Agent Targeting. https://www.researchsquare.com/article/rs-6232250/v1
Latif, R., & Nawaz, T. (2025). Medicinal plants and human health: a comprehensive review of bioactive compounds, therapeutic effects, and applications. In Phytochemistry Reviews (Vol. 25, Issue 3). Springer Netherlands. https://doi.org/10.1007/s11101-025-10194-7
Li, Y., Wan, C., Li, F., Xin, G., Wang, T., Zhou, Q., Wen, T., Li, S., Chen, X., & Huang, W. (2025). Indirubin attenuates sepsis by targeting the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways in macrophages. Frontiers in Pharmacology, 16(March), 1–20. https://doi.org/10.3389/fphar.2025.1542061
Lotts, T., Kabrodt, K., Hummel, J., Binder, D., Schellenberg, I., Ständer, S., & Agelopoulos, K. (2020). Isatis tinctoria l.-derived petroleum ether extract mediates antiinflammatory effects via inhibition of interleukin-6, interleukin-33 and mast cell degranulation. Acta Dermato-Venereologica, 100(10), 1–9. https://doi.org/10.2340/00015555-3476
Manjusha, P., Rai, S., & More, A. B. (2026). International Journal of Ayurvedic Medicine Mapping the Landscape of Psoriasis Research in Traditional Medicine : A VOS viewer-Based Bibliometric Review ( 1980 – 2024 ). International Journal of Ayurvedic Medicine, 17(1), 25–31. https://doi.org/https://doi.org/ 10.47552/ ijam.v17i1.6558
Matsuno, Y., Torisu, T., Umeno, J., Shibata, H., Hirano, A., Fuyuno, Y., Okamoto, Y., Fujioka, S., Kawasaki, K., Moriyama, T., Nagasue, T., Zeze, K., Hirakawa, Y., Kawatoko, S., Koga, Y., Oda, Y., Esaki, M., & Kitazono, T. (2022). One-year clinical efficacy and safety of indigo naturalis for active ulcerative colitis: A real-world prospective study. Intestinal Research, 20(2), 260–268. https://doi.org/10.5217/ir.2021.00124
Mishra DN, Gomare KS, S. S. (2020). GC-MS Analysis and Phytochemical Screening of Indigofera tinctoria (Linn.) Leaf Extract Characterizing its Medicinal Use. International Journal of Ayurvedic Medicine, 11(2), 289–299. https://doi.org/10.47552/ijam.v11i2.1540
Muda, I., Prastowo, J., Nurcahyo, W., & Sarmin, S. (2021). Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia. Veterinary World, 14(5), 1272–1278. https://doi.org/10.14202/vetworld.2021.1272-1278
N, M. D., S, G. K., & V, S. S. (2020). GC-MS Analysis and Phytochemical Screening of Indigofera tinctoria (Linn.) Leaf Extract Characterizing its Medicinal Use. International Journal of Ayurvedic Medicine, 11(2), 289–299. https://doi.org/10.47552/ijam.v11i2.1540
Page, M. J., McKenzie, J. E., Bossuyt, P. M., Boutron, I., Hoffmann, T. C., Mulrow, C. D., Shamseer, L., Tetzlaff, J. M., Akl, E. A., Brennan, S. E., Chou, R., Glanville, J., Grimshaw, J. M., Hróbjartsson, A., Lalu, M. M., Li, T., Loder, E. W., Mayo-Wilson, E., McDonald, S., … Moher, D. (2021). The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. Bmj, 372. https://doi.org/10.1136/bmj.n71
Parham ve ark. (2020). Antioxidant, antimicrobial and antiviral properties of herbal materials, Antioxidants, www.mdpi.com/journal/antioxidants. Antioxidants, 9(12), 1–36.
Patwardhan, B., & Aswar, U. (2025). Harnessing the potential of ethnopharmacology for future medicines. Journal of Ethnopharmacology, 353(120359). https://doi.org/10.1016/j.jep.2025.120359
Pirintsos, S., Panagiotopoulos, A., Bariotakis, M., Daskalakis, V., Lionis, C., Sourvinos, G., Karakasiliotis, I., Kampa, M., & Castanas, E. (2022). From Traditional Ethnopharmacology to Modern Natural Drug Discovery : A Methodology Discussion and Specific Examples. Molecules, 27(4060), 1–18. https://doi.org/https://doi.org/10.3390/molecules27134060
Qi-yue, Y., Ting, Z., Ya-nan, H., Sheng-jie, H., Xuan, D., Li, H., & Chun-guang, X. (2020). From natural dye to herbal medicine: a systematic review of chemical constituents, pharmacological effects and clinical applications of indigo naturalis. Chinese Medicine (United Kingdom), 15(1), 1–13. https://doi.org/10.1186/s13020-020-00406-x
Rao, P. S., Fatima, N., Siddiqui, M. H., Habib, K., Mohasin, M., Haq, W., Chinyo, M., Zaidi, H., Shah, Y. A., Mazeed, A., & Siddiqui, S. (2025). Indigofera tinctoria: the blue gold of India’s sustainable future. Discover Sustainability, 6(1). https://doi.org/10.1007/s43621-025-01120-0
Reza, M., & Khouzani, Z. (2022). Assessing Indigofera tinctoria L. as a forgotten medicinal industrial plant and the importance of its revitalization for the sustainability of Iran’s agricultural ecosystems. Central Asian Journal of Environmental Science and Technology Innovation, 3(2), 32–39.
Roy, A., Khan, A., Ahmad, I., Alghamdi, S., Rajab, B. S., Babalghith, A. O., Alshahrani, M. Y., Islam, S., & Islam, M. R. (2022). Flavonoids a Bioactive Compound from Medicinal Plants and Its Therapeutic Applications. BioMed Research International, 2022. https://doi.org/10.1155/2022/5445291
Sarkar, B., Biswas, P., Adhikari, S., Roy, D., Behera, B. K., Madhu, N. R., & Erfani, H. (2025). Pharmacological Uses of New Bioactive Compounds from Medicinal Plants. In A Basic Handbook of Science, Technology and Innovation for Inclusive Development [Volume: 2] (Issue June). https://doi.org/10.52756/bhstiid.2025.e02.011
Shahidi, F., & Samarasinghe, A. (2025). How to assess antioxidant activity? Advances, limitations, and applications of in vitro, in vivo, and ex vivo approaches. In Food Production, Processing and Nutrition (Vol. 7, Issue 1). BioMed Central. https://doi.org/10.1186/s43014-025-00326-z
Shivani, S. (2025). Antimicrobial Activity and Phytochemical Analysis of Indigofera Tinctoria. International Journal of Advanced Research, 13(05), 50–66. https://doi.org/10.21474/ijar01/20876
Speranza, J., Miceli, N., Taviano, M. F., Ragusa, S., Kwiecień, I., Szopa, A., & Ekiert, H. (2020). Isatis tinctoria L. (woad): A review of its botany, ethnobotanical uses, phytochemistry, biological activities, and biotechnological studies. Plants, 9(3). https://doi.org/10.3390/plants9030298
Sun, Q., Leng, J., Tang, L., Wang, L., & Fu, C. (2021). A Comprehensive Review of the Chemistry, Pharmacokinetics, Pharmacology, Clinical Applications, Adverse Events, and Quality Control of Indigo Naturalis. Frontiers in Pharmacology, 12(May), 1–13. https://doi.org/10.3389/fphar.2021.664022
Thamizhoviya, G. (2025). Global Integration of Traditional and Modern Medicine: Policy Developments, Regulatory Frameworks, and Clinical Integration Model. Future Integrative Medicine, 4(3), 180–190. https://doi.org/10.14218/fim.2025.00033
Thanuja, T. T., Sonia, N. S., & Nair, S. (2026). Indigofera tinctoria L .: A Comprehensive Review on Botany , Phytochemistry , Agronomy and Pharmacological Perspectives. Journal of Advances in Biology & Biotechnology, 29(3), 379–388. https://doi.org/https://doi.org/10.9734/jabb/2026/v29i33743
Tuasha, N., Fekadu, S., & Deyno, S. (2023). Prevalence of herbal and traditional medicine in Ethiopia: a systematic review and meta-analysis of 20-year studies. Systematic Reviews, 12(1). https://doi.org/10.1186/s13643-023-02398-9
Wang, J. (2024). Indirubin induces apoptosis in ovarian cancer cells via the mitochondrial pathway. American Journal of Translational Research, 16(10), 6119–6129. https://doi.org/10.62347/iofy5604
Wang, X., Anwar, T., Qureshi, H., El-Beltagi, H. S., Sehar, Z., Solieva, D., Azizov, B., Rebouh, N. Y., Abbasov, M. A., Yakubov, F., & Alomran, M. M. (2025). Plant-based traditional remedies and their role in public health: ethnomedicinal perspectives for a growing population. Journal of Health, Population and Nutrition, 44(1). https://doi.org/10.1186/s41043-025-01036-5
Wang, Y., Khan, F. A., Siddiqui, M., Aamer, M., Lu, C., Atta-ur-Rahman, Atia-tul-Wahab, & Choudhary, M. I. (2021). The genus Schefflera: A review of traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology, 279. https://doi.org/10.1016/j.jep.2020.113675
Xie, X., Liu, C., Liu, X., Hou, L., Zhang, Y., Huang, L., Peng, W., & Liu, Y. (2026). Indirubin treatment was associated with modulation of the PI3K/AKT and MAPK pathways and induction of apoptosis and autophagy. Scientific Reports, 1–10. https://doi.org/10.1038/s41598-026-35382-z
Zahra, M., Abrahamse, H., & George, B. P. (2024). Flavonoids: Antioxidant Powerhouses and Their Role in Nanomedicine. Antioxidants, 13(8). https://doi.org/10.3390/antiox13080922
Zourob, J., & Lio, P. (2026). Indigo Naturalis in Dermatology : Current Evidence and Clinical Applications.
