Exploration of Endophytic Bacteria from Katuk Plants (Sauropus androgynus L.)
DOI:
https://doi.org/10.51574/hayyan.v1i3.2442Keywords:
Endophytic Bacteria, Plant Pathogen, AntibacterialAbstract
This study aimed to explore and identify endophytic bacteria from katuk plants (Sauropus androgynus L.) and evaluate their potential as biological control agents. A total of 14 bacterial isolates were successfully obtained from the roots, stems, and leaves of katuk plants. Safety assessments were conducted through hypersensitivity response (HR) and hemolysis (HL) tests. The HR test on tobacco plants indicated that all isolates were non-pathogenic, as no necrotic symptoms were observed. However, the hemolysis test revealed two isolates capable of forming α-hemolysis zones on blood agar, suggesting potential risks to mammals. Antibacterial activity against the plant pathogen Ralstonia solanacearum was evaluated using spot inoculation methods. The results showed that none of the isolates were able to inhibit the growth of R. solanacearum. Although the isolates may produce antibacterial compounds, the amounts are likely insufficient or involve unidentified active compounds. Further studies are needed to fully characterize these isolates and explore their potential applications in sustainable agriculture.
References
Bacon CW, Hinton DM. 2007. Bacterial Endophytes: the Endophytic Niche, its Occupants, and its Utility. Berlin (DE): Springer.
Fatimah S, Yuliana P, Aris M. 2010. Efektifitas Ekstrak Daun Katuk (Sauropus Androgynus L.) Dalam Menghambat Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro ipi179205. Stikes Guna Bangsa, Yogyakarta.
Hariprasad P, Divakara S, Niranjana S. 2011. Isolation and characterization of chitinolytic Rhizobacteria for the management of Fusarium Wilt in Tomato. Crop Protection. 30(12):1606-1612.doi: 10.1016/j.cropro. 2011.02.032.
Klement Z. 1982. Hypersensitivity defense reaction of plants to pathogens. Annual Review Phytopatology. 5(3):17-44.doi:10.1146/annurev.py.05.0 90167.000313.
Khusnan, Oktavia S, Soegiyono. 2008. Isolation and phenotipical characterization of Staphylococcus aureus isolated from slaughtered house waste and carcass of chicken. Jurnal Veteriner 9(1):45-51.
Klement Z, Rudolph K, Sands D. 1990. Methods In Phytobacteriology. Hungaria (HU): Akademia Kiado.
Koomnok C, Teaumroong N, Rerkasem B, Lumyong S. 2007. Diazotrop endophytic bacteria in cultivated and wild rice in Thailand. Science Asia 33: 429-435.
Lamb TG, Tonkyn DW, Kluepfel DA. 1996. Movement of Pseudomonas aureofaciens from the rhizosphere to aerial plant tissue. Can. J.Microbiol. 42:1112–1120.
Munif A, Hallmann J, Sikora RA. 2013. The influence of endophytic bacteria on Meloidogyne incognita infection and tomato plant growth. Journal International Society for Southeast Asian Agricultural Sciences 19(2):68- 74.
Munif A, Sulistiawati I. 2014. Pengelolaan penyakit kuning pada tanaman lada oleh petani di wilayah Bangka. Jurnal Fitopatologi Indonesia. 10(1):8-16.doi: 10.14692/jfi.10.1.8.
Oktafiyanto M.F. The Potential of Endophytic Bacteria from Mangrove Plants as a Biocontrol Agent Against Ralstonia solanacearum and Meloidogyne spp. in Tomato Plants. Repository IPB. Diakses pada 29 Juni 2018.
Pratiwi RS, Susanto TE, Sutrisno A. 2014. Enzim kitinase dan aplikasi di bidang industri. Jurnal Pangan dan Agroindustri 3(3):878-887.
Salasia SIO, Khusnan Z, Lammler C, Zschock M. 2004. Comparative studies on pheno-and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in Central Java in Indonesia and Hesse in Germany. Journal of Veterinary Science (2):103-109.
Schulz B, Boyle C. 2006. What Are Endophytes. Berlin (DE): Springer.
