EVALUASI WAKTU RETENSI, FAKTOR KAPASITAS, DAN LINEARITAS SENYAWA PAH MENGGUNAKAN GC-FID
DOI:
https://doi.org/10.51574/illea.v2i2.5291Keywords:
Polisiklik Aromatik Hidrokarbon, Kromatografi Gas, Sedimen, Polusi LingkunganAbstract
Polisiklik Aromatik Hidrokarbon (Polycyclic Aromatic Hydrocarbons/PAHs) merupakan kelompok senyawa organik yang bersifat toksik, persisten, serta berpotensi menimbulkan efek mutagenik dan karsinogenik bagi organisme hidup. Keberadaan PAH di lingkungan perairan umumnya berasal dari aktivitas antropogenik, seperti pembakaran bahan bakar fosil, aktivitas industri, dan tumpahan minyak. Penelitian ini bertujuan untuk mengevaluasi kinerja metode kromatografi gas dalam analisis senyawa PAH melalui penentuan parameter waktu retensi, faktor kapasitas, jumlah pelat teoritis, dan linearitas metode. Analisis dilakukan menggunakan Gas Chromatography-Flame Ionization Detector (GC-FID) terhadap tujuh senyawa standar PAH, yaitu Naphtalene, Acenaphthene, Phenanthrene, Fluoranthene, Pyrene, Benzo(a)anthracene, dan Perylene. Hasil penelitian menunjukkan bahwa seluruh senyawa PAH dapat dipisahkan dengan baik dengan waktu retensi berkisar antara 4,83 hingga 24,05 menit. Nilai faktor kapasitas (k') berada pada rentang 0,799–6,240, sedangkan jumlah pelat teoritis menunjukkan efisiensi kolom yang baik. Uji linearitas pada rentang konsentrasi 0,5–20 ppm menghasilkan koefisien korelasi (R) sebesar 0,991–0,999 yang menunjukkan hubungan linear yang sangat kuat antara konsentrasi analit dan respons detektor. Berdasarkan hasil tersebut, metode GC-FID yang digunakan memiliki performa yang baik dan layak diterapkan untuk analisis kuantitatif senyawa PAH pada sampel lingkungan, khususnya sedimen laut
References
Abdel-Shafy, H. I., & Mansour, M. S. M. (2016). A review on polycyclic aromatic hydrocarbons: Source, environmental impact, effect on human health and remediation. Egyptian Journal of Petroleum, 25(1), 107–123. https://doi.org/10.1016/J.EJPE.2015.03.011
Abdulai, P. M., Bede-Ojimadu, O., Onyena, A. P., Frazzoli, C., Mogborukor, N. A., Ekhator, O. C., Udom, G. J., Nwanaforo, E., & Orisakwe, O. E. (2025). Public Health Effects of Polycyclic Aromatic Hydrocarbons Exposure Through Air, Water, Soil, and Food in Ghana: Possible Economic Burden. Environmental Health Insights, 19, 11786302251343768. https://doi.org/10.1177/117863022513437
Abhishek, Chakkaravarthi, S., & Agarwal, T. (2025). Fish consumption patterns and health risk assessment of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in fried and grilled fish products and mitigation strategies. Toxicology Reports, 14, 101953. https://doi.org/10.1016/J.TOXREP.2025.101953
Al Solami, L. (2025). Polycyclic aromatic hydrocarbon levels in marine fish samples from the central Red Sea, Saudi Arabia. Egyptian Journal of Aquatic Research, 51(4), 463–468. https://doi.org/10.1016/J.EJAR.2025.08.003
Baran, A., Tarnawski, M., Urbański, K., Klimkowicz-Pawlas, A., & Spałek, I. (2017). Concentration, sources and risk assessment of PAH in bottom sediments. Environmental Science and Pollution Research International, 24(29), 23180. https://doi.org/10.1007/S11356-017-9944-Y
Boström, C.-E., Gerde, P., Hanberg, A., Jernström, B., Johansson, C., Kyrklund, T., Rannug, A., Törnqvist, M., Victorin, K., & Westerholm, R. (2002). Cancer Risk Assessment, Indicators, and Guidelines for Polycyclic Aromatic Hydrocarbons in the Ambient Air. Environmental Health Perspectives, 110(s3), 451–489. https://doi.org/10.1289/EHP.02110S3451
Cheng, J. O., Chu, C. H., Chang, C. W., Chen, T. H., Hsieh, C. Y., & Ko, F. C. (2025). Bioaccumulation and Trophic Transfer of Polycyclic Aromatic Hydrocarbons (PAH) in the Planktonic Base of the Aquatic Food Web. Archives of Environmental Contamination and Toxicology, 89(4), 528–537. https://doi.org/10.1007/S00244-025-01157-2
Ding, C., Ni, H. G., & Zeng, H. (2013). Human exposure to parent and halogenated polycyclic aromatic hydrocarbons via food consumption in Shenzhen, China. Science of the Total Environment, 443, 857–863. https://doi.org/10.1016/j.scitotenv.2012.11.018
Dong, Y., Yan, Z., Wu, H., Zhang, G., Zhang, H., & Yang, M. (2021). Polycyclic Aromatic Hydrocarbons in Sediments from Typical Algae, Macrophyte Lake Bay and Adjoining River of Taihu Lake, China: Distribution, Sources, and Risk Assessment. Water 2021, Vol. 13, Page 470, 13(4), 470. https://doi.org/10.3390/W13040470
Du, W., Jiang, S., Lei, Y., Wang, J., Cui, Z., Xiang, P., Chang, Z., Duan, W., Shen, G., Qin, Y., Pan, B., & Yu, Y. (2025). Occurrence, formation mechanism, and health risk of polycyclic aromatic hydrocarbons in barbecued food. Ecotoxicology and Environmental Safety, 293. https://doi.org/10.1016/j.ecoenv.2025.118046
Ferrante, M., Zanghì, G., Cristaldi, A., Copat, C., Grasso, A., Fiore, M., Signorelli, S. S., Zuccarello, P., & Oliveri Conti, G. (2018). PAH in seafood from the Mediterranean Sea: An exposure risk assessment. Food and Chemical Toxicology : An International Journal Published for the British Industrial Biological Research Association, 115, 385–390. https://doi.org/10.1016/J.FCT.2018.03.024
Fogaça, F. H. dos S., Melo, P. T. da S., Massone, C. G., Carreira, R. da S., Ramos, L. R. V., & Torres, J. P. M. (2025). Polycyclic Aromatic Hydrocarbons in Seafood: Occurrence, Trophic Bioaccumulation, and Human Health Risks. Fishes, 10(11), 591. https://doi.org/10.3390/FISHES10110591/S1
Han, B., Cui, D., Liu, A., Li, Q., & Zheng, L. (2021). Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAH) in surface sediments from Daya Bay, South China. Environmental Science and Pollution Research International, 28(20), 25858–25865. https://doi.org/10.1007/S11356-020-11956-W
Jinadasa, B. K. K. K., Monteau, F., & Fowler, S. W. (2020). Review of polycyclic aromatic hydrocarbons (PAH) in fish and fisheries products; a Sri Lankan perspective. Environmental Science and Pollution Research International, 27(17), 20663–20674. https://doi.org/10.1007/S11356-020-08305-2
Kim, K. H., Jahan, S. A., Kabir, E., & Brown, R. J. C. (2013). A review of airborne polycyclic aromatic hydrocarbons (PAH) and their human health effects. Environment International, 60, 71–80. https://doi.org/10.1016/j.envint.2013.07.019
Kuhn, A. V., Pont, G. D., Cozer, N., & Sadauskas-Henique, H. (2024). The concentrations of polycyclic aromatic hydrocarbons in fish: A systematic review. Marine Pollution Bulletin, 198, 115778. https://doi.org/10.1016/J.MARPOLBUL.2023.115778
